{"product_id":"brefeldin-a-solution-1000x","title":"Brefeldin A Solution 1000X","description":"\u003cp\u003eFungal metabolite with the property to inhibit intracellular protein transport. It is a reversible inhibitor of protein translocation from the endoplasmic reticulum (ER) to the Golgi apparatus. It blocks binding of ADP-ribosylation factor to the Golgi apparatus and inhibits GDP-GTP exchange. Studies show prolonged treatment with BFA induce cell apoptosis. Brefeldin A-mediated apoptosis has been observed in human tumor cells.\u003c\/p\u003e\r\n\u003cp\u003eBrefeldin A working concentrations and length of treatment can vary depending on desired effect. Inhibiton of ER to Golgi trafficking was observed as low as 100 ng\/ml and apoptosis was observed with prolonged treatment at 10 μg\/ml.\u003cbr\u003e \u003cbr\u003e \u003cstrong\u003eSynonym: \u003c\/strong\u003e γ-4-Dihdyroxy-2-(6-hydroxy-1- heptenyl)-4-cyclopentanecrotonic\u003cbr\u003e \u003cbr\u003e \u003cstrong\u003eFormulation: \u003c\/strong\u003e Brefeldin A Solution is supplied as a 1000X in DMSO, which should be diluted to 1X in cell culture medium. It is recommended that cells are cultured with brefeldin A for ≤ 24 hours, as this can become toxic for cell viability.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eConcentration: \u003c\/strong\u003e 5.0 mg\/ml\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eStorage \u0026amp; Handling: \u003c\/strong\u003e Store the Brefeldin A Solution (1,000X) at 4°C. Completely thaw the solution before use, DMSO freezes at 4°C. We recommend aliquotting the reagent into smaller volumes to avoid repeated freeze-thawing\u003c\/p\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\n\u003cstrong\u003eAppearance: \u003c\/strong\u003eLiquid\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cstrong\u003eBoiling Point: \u003c\/strong\u003e492.68 °C at 760 mmHg\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cstrong\u003eDensity: \u003c\/strong\u003e1.11 g\/cm3\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cstrong\u003eMelting Point: \u003c\/strong\u003e204-205 °C\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cstrong\u003eRTECS Number: \u003c\/strong\u003eGY8410000\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"bioPLUS","offers":[{"title":"1 mL","offer_id":47711065964716,"sku":"vus1793007943927","price":107.23,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0691\/5132\/6380\/files\/brefeldin-a-solution-1000x_1.png?v=1793007951","url":"https:\/\/www.meadowterrace.shop\/products\/brefeldin-a-solution-1000x","provider":"My Store","version":"1.0","type":"link"}